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Proceedings Paper

Cellulose protein films for highly specific evanescent wave immunosensors
Author(s): Frank Loescher; Andreas Hartmann; J. Ueberfeld; Thomas Ruckstuhl; Daniel Bock; Thomas Jaworek; Gerhard Wegner; Stefan Seeger
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Paper Abstract

The immobilization techniques presented represent an innovative method for the highly specific determination of antigens and other proteins in a very short time and may be a useful tool for the industrial preparation of highly sensitive biosensors. Non amphiphilic alkylated cellulose films are used as matrices to reduce non-specific interactions with biomolecules. These films are transferred onto waveguides by using the Langmuir-Blodgett- technique. After photochemical stabilization the cellulose films serve as excellent matrices for the immobilization of proteins at high density. Furthermore, one-step immobilization of a mixed photopolymer antibody film with a continuously operating LB-trough is demonstrated. The activity and specificity of immobilized antibodies and streptavidin is controlled by an enzyme-linked immunosorbent assay (ELISA) and an evanescent wave immunosensor.

Paper Details

Date Published: 26 November 1996
PDF: 11 pages
Proc. SPIE 2928, Biomedical Systems and Technologies, (26 November 1996); doi: 10.1117/12.259974
Show Author Affiliations
Frank Loescher, Univ. Heidelberg (Germany)
Andreas Hartmann, Univ. Heidelberg (Germany)
J. Ueberfeld, Univ. Heidelberg (Germany)
Thomas Ruckstuhl, Univ. Heidelberg (Germany)
Daniel Bock, Univ. Heidelberg (Germany)
Thomas Jaworek, Max-Planck-Institut fuer Polymerforschung (Germany)
Gerhard Wegner, Max-Planck-Institut fuer Polymerforschung (Germany)
Stefan Seeger, Univ. Heidelberg (Germany)

Published in SPIE Proceedings Vol. 2928:
Biomedical Systems and Technologies
Nathan I. Croitoru; Martin Frenz; Terence A. King; Riccardo Pratesi; Anna Maria Verga Scheggi; Stefan Seeger; Otto S. Wolfbeis, Editor(s)

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