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Proceedings Paper

Corneal collagen morpho-mechanics characterized by correlative optical microscopies
Author(s): Raffaella Mercatelli; Sara Mattana; Laura Capozzoli; Fulvio Ratto; Francesca Rossi; Roberto Pini; Daniele Fioretto; Francesco S. Pavone; Silvia Caponi; Riccardo Cicchi
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Paper Abstract

Tissue morpho-mechanics is gaining an increasing relevance in various fields, including biology, medicine, pathology, tissue engineering, and regenerative medicine, since it targets the relationship between morphological features and mechanical properties in biological tissues, which plays an important role in various biological processes including metastasis, wound healing and tissue regeneration. In particular, in every biological tissue, morphological, biochemical and mechanical properties are tightly connected and they influence each other in a correlative manner. For this reason, a correlative approach employing multiple techniques is ideal for targeting tissue morpho-mechanics with an optical approach. Here we report a correlative study performed by optical microscopies, disclosing the supramolecular collagen morphology correlated with its biomechanical and biochemical analyses. In particular, using human corneal tissue as a benchmark, we correlate Second-Harmonic Generation maps with mechanical and biochemical imaging obtained by Brillouin and Raman micro-spectroscopy, demonstrating that the peculiar mechanical functionality of so-called sutural lamellae originates from their distinctive supramolecular organization. A theoretical model based on the ultrastructural symmetry of corneal lamellar domains provides the interpretation of the experimental data at the molecular scale. The proposed methodology opens the way to the non-invasive assessment of tissue morpho-mechanics and holds the potential to be applicable to a broad range of biological and synthetic materials.

Paper Details

Date Published: 1 April 2020
PDF: 8 pages
Proc. SPIE 11359, Biomedical Spectroscopy, Microscopy, and Imaging, 1135908 (1 April 2020); doi: 10.1117/12.2555703
Show Author Affiliations
Raffaella Mercatelli, National Institute of Optics, National Reasearch Council (Italy)
Aerospazio Tecnologie s.r.l. (Italy)
Sara Mattana, National Institute of Optics, National Reasearch Council (Italy)
Univ. of Florence (Italy)
Laura Capozzoli, Institute of Chemistry of Organometallic Compounds, National Research Council (Italy)
Fulvio Ratto, Institute of Applied Physics "Nello Carrara", National Research Council (Italy)
Francesca Rossi, Institute of Applied Physics "Nello Carrara", National Research Council (Italy)
Roberto Pini, Institute of Applied Physics "Nello Carrara", National Research Council (Italy)
Daniele Fioretto, Univ. of Perugia (Italy)
CEMIN (Italy)
Francesco S. Pavone, National Institute of Optics, National Reasearch Council (Italy)
Univ. of Florence (Italy)
European Lab. for Non-Linear Spectroscopy (Italy)
Silvia Caponi, CEMIN (Italy)
Univ. of Perugia (Italy)
Riccardo Cicchi, National Institute of Optics, National Reasearch Council (Italy)
European Lab. for Non-Linear Spectroscopy (Italy)


Published in SPIE Proceedings Vol. 11359:
Biomedical Spectroscopy, Microscopy, and Imaging
Jürgen Popp; Csilla Gergely, Editor(s)

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