Share Email Print

Proceedings Paper

Fiber-based label-free D-dimer detection for early diagnosis of venous thromboembolism
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

D-dimer is a useful diagnostic biomarker for deep vein thrombosis or pulmonary embolism, collectively referred to as venous thromboembolism (VTE). The ability to detect in real-time the amount of D-dimer with a fast and reliable method is a key step to anticipate the appearance of these diseases. The combination of fiber-optic–based platforms for biosensing with the nanotechnologies is opening up the chance for the development of in situ, portable, lightweight, versatile, reliable and high-performance optical sensing devices towards lab-on-fiber technology. The generation of lossy mode resonances (LMRs) by means of the deposition of nm-thick absorbing metal-oxide films on special geometric-modified fibers allows measuring precisely and accurately surface refractive index changes, which are due to the binding interaction between a biological recognition element and the analyte under investigation. This approach enhances the light-matter interaction in a strong way, thus turning out to be more sensitive compared to other optical technology platforms, such as fiber gratings or surface plasmon resonance. Here, the results of a highly specific and sensitive biosensor for the detection of D-dimer based on LMR in fiber-optics are presented by monitoring in real-time the shift of the LMR related to the biomolecule interactions thanks to a conventional wavelength-interrogation system and an ad-hoc developed microfluidics. A detection limit of 100 ng/mL, a value 5-fold below the clinical cutoff value, has been attained for D-dimer spiked in human serum. The comparison of the results achieved with proteomics-based methodologies, which allows for the identification of betaand gamma-chains of fibrinogen, demonstrates the ability of our platform to specifically (<90%) recognize D-dimer.

Paper Details

Date Published: 1 April 2020
PDF: 7 pages
Proc. SPIE 11354, Optical Sensing and Detection VI, 113540S (1 April 2020); doi: 10.1117/12.2555693
Show Author Affiliations
P. Zubiate, Univ. Pública de Navarra (Spain)
A. Urrutia, Univ. Pública de Navarra (Spain)
C. R. Zamarreño, Institute of Smart Cities, Univ. Pública de Navarra (Spain)
J. Fernández-Irigoyen, Complejo Hospitalario de Navarra, Univ. Pública de Navarra (Spain)
A. Giannetti, Istituto di Fisica Applicata "Nello Carrara" (Italy)
F. Baldini, Istituto di Fisica Applicata "Nello Carrara" (Italy)
S. Díaz, Univ. Pública de Navarra (Spain)
I. R. Matias, Institute of Smart Cities, Univ. Pública de Navarra (Spain)
F. J. Arregui, Univ. Pública de Navarra (Spain)
E. Santamaría, Navarrabiomed, Complejo Hospitalario de Navarra, Univ. Pública de Navarra (Spain)
I. Del Villar, Institute of Smart Cities, Univ. Pública de Navarra (Spain)
F. Chiavaioli, Istituto di Fisica Applicata "Nello Carrara" (Italy)

Published in SPIE Proceedings Vol. 11354:
Optical Sensing and Detection VI
Francis Berghmans; Anna G. Mignani, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?