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Proceedings Paper

A new macro-imager based on Tpx3Cam optical camera for PLIM applications
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Paper Abstract

The recently designed Tpx3Cam camera based PLIM (Phosphorescence Lifetime IMaging) macro-imager was tested using an array of phosphorescent chemical and biological samples. A series of sensor materials prepared by incorporating the phosphorescent O2-sensitive dye, PtBP, into five polymers with different O2 permeability were imaged along with several commercial and non-commercial sensors based on PtBP and PtOEPK dyes. The PLIM images showed good lifetime contrast between the different materials, and phosphorescence lifetime values obtained were consistent with those measured by alternative methods. A panel of live tissues samples stained with PtBP based nanoparticle probe were also prepared and imaged under resting conditions and upon inhibition of respiration. The macro-imager showed promising results as a tool for PLIM of O2 in chemical and biological samples.

Paper Details

Date Published: 1 April 2020
PDF: 8 pages
Proc. SPIE 11359, Biomedical Spectroscopy, Microscopy, and Imaging, 113591N (1 April 2020); doi: 10.1117/12.2555387
Show Author Affiliations
Rajannya Sen, Univ. College Cork (Ireland)
Alexander Zhdanov, Univ. College Cork (Ireland)
Liisa M. Hirvonen, The Univ. of Western Australia (Australia)
Peter Svihra, Czech Technical Univ. in Prague (Czech Republic)
The Univ. of Manchester (United States)
Stefan Andersson-Engels, Tyndall National Institute (Ireland)
Andrei Nomerotski, Brookhaven National Lab. (United States)
Dmitri Papkovsky, Univ. College Cork (Ireland)
Tyndall National Institute (Ireland)

Published in SPIE Proceedings Vol. 11359:
Biomedical Spectroscopy, Microscopy, and Imaging
Jürgen Popp; Csilla Gergely, Editor(s)

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