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Proceedings Paper

Reduction in data acquisition for resolution improvement in structured illumination digital holographic microscopy
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Paper Abstract

Over the last years structured illumination digital holographic microscopy (SI-DHM) has been experimentally proved to double the resolution limit in conventional DHM. In SI-DHM, the underlying specimen is illuminated using a spatially varying structured illumination (SI) pattern, which enables super-resolution (SR) images to be retrieved using the proper computational reconstruction process. All these reconstruction methods require the acquisition of at least a couple phase-shifted DHM images. In particular, for a pure sinusoidal pattern, there is a need of recording two phase-shifted DHM images per orientation of the pattern (e.g., 6 images per isotropic SR improvement). Taking advantage of the simultaneous recording of the virtual (e.g., conjugated) image in the raw DHM image, here we present a novel computational method to reconstruct an isotropic SR image using one acquisition per pattern’s orientation (e.g. total 3 images per isotropic improvement). Because our proposed method shows a 50% reduction in the data acquisition and, therefore, acquisition time, we believe that our method should increase the utility of SI-DHM in live-cell imaging. We have validated our method using simulated and results.

Paper Details

Date Published: 27 April 2020
PDF: 8 pages
Proc. SPIE 11402, Three-Dimensional Imaging, Visualization, and Display 2020, 114020R (27 April 2020); doi: 10.1117/12.2555091
Show Author Affiliations
R. Castaneda, The Univ. of Memphis (United States)
T. O'Connor, Univ. of Connecticut (United States)
Ana Doblas, The Univ. of Memphis (United States)
B. Javidi, Univ. of Connecticut (United States)

Published in SPIE Proceedings Vol. 11402:
Three-Dimensional Imaging, Visualization, and Display 2020
Bahram Javidi; Manuel Martínez-Corral; Osamu Matoba; Adrian Stern, Editor(s)

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