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Proceedings Paper

Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope
Author(s): Johanna Trägårdh; Tomáš Pikálek; Mojmír Šerý; Denis Akimov; Tobias Meyer; Jürgen Popp; Tomáš Cižmár
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Paper Abstract

Advanced wavefront-shaping methods can be used to transform a simple multimode fiber into an ultra-thin laser scanning microscope. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fiber endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial, 125 μm diameter, 0.29 NA, GRIN fiber as the endoscopic probe. Wavefront shaping on a spatial light modulator is used to create a focus, where the 1-2 ps long pump and Stokes pulses are overlapped in time, which is scanned behind the fiber facet across the sample. The chemical selectivity is demonstrated by imaging 2 μm polystyrene and 2.5 μm PMMA beads with per pixel integration time as low as 1 ms for epi-detection. Epi-detection through the fiber is possible despite the fact that the CARS signal is emitted mainly in the forward direction, away from the fiber facet. Detecting the back-scattered signal from the underlying tissue, requires a large detector aperture to be efficient. By detecting through both the core and the cladding of the fiber, we obtain sufficient detection efficiency.

Paper Details

Date Published: 1 April 2020
PDF: 9 pages
Proc. SPIE 11359, Biomedical Spectroscopy, Microscopy, and Imaging, 1135907 (1 April 2020); doi: 10.1117/12.2555080
Show Author Affiliations
Johanna Trägårdh, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Tomáš Pikálek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Mojmír Šerý, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Denis Akimov, Leibniz Institute of Photonic Technology (Germany)
Tobias Meyer, Leibniz Institute of Photonic Technology (Germany)
Friedrich Schiller Univ. of Jena (Germany)
Abbe Ctr. of Photonics (Germany)
Jürgen Popp, Leibniz Institute of Photonic Technology (Germany)
Friedrich Schiller Univ. of Jena (Germany)
Abbe Ctr. of Photonics (Germany)
Tomáš Cižmár, Institute of Scientific Instruments (Czech Republic)
Friedrich Schiller Univ. of Jena (Germany)
Abbe Ctr. of Photonics (Germany)


Published in SPIE Proceedings Vol. 11359:
Biomedical Spectroscopy, Microscopy, and Imaging
Jürgen Popp; Csilla Gergely, Editor(s)

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