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The role of photochemical transformations of tetrahydrobiopterin in the pathogenesis and phototherapy of vitiligo
Author(s): Alexey S. Nizamutdinov; Taisia A. Telegina; Andrey A. Buglak; Elena V. Lukinova; Eduard I. Madirov; Yuliya L. Vechtomova; Mikhail S. Kritsky; Vadim V. Semashko
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Paper Abstract

Melanogenesis disorder leads to several pathologies, including vitiligo. Tetrahydrobiopterin (H4Bip) as the phenylalanine 4-hydroxylase coenzyme catalyzes the oxidation of phenylalanine to tyrosine (a melanin precursor). H4Bip is easily oxidized by oxygen in vivo and in vitro. Vitiligo is accompanied by three-fivefold increased de-novo synthesis of H4Bip, its excess and its further oxidation are essential factors in the pathogenesis of vitiligo. We have demonstrated that pterin products of H4Bip autoxidation (dihydropterin (H2Ptr), dihydroxanthopterin and pterin) predominate over biopterin products (dihydrobiopterin (H2Bip) and biopterin). It was shown that ultraviolet (UV) irradiation accelerates the autoxidation while the products of oxidative degradation of H4Bip act as photosensitizers. Photosensitized oxidation of H4Bip can contribute to the pathogenesis of vitiligo. The main distinguishing feature of UV photooxidation of H4Bip from autoxidation was the formation of dihydropterin (Н2Ptr)2 and dihydrobiopterin (Н2Bip)2 dimers. Here we reported on the dependences of the photodimerization reaction on the wavelength and intensity of radiation using xenon lamps and UV tunable lasers as sources of UV radiation. It was shown that UV irradiation with a laser is more efficient than that with xenon lamp. It was established that the greatest number of dimers were formed by irradiating the H4Bip solution by radiation with a wavelength in the range 308-312 nm. The data obtained are discussed in the context of UVB narrowband vitiligo phototherapy.

Paper Details

Date Published: 11 December 2019
PDF: 7 pages
Proc. SPIE 11322, XIV International Conference on Pulsed Lasers and Laser Applications, 1132207 (11 December 2019); doi: 10.1117/12.2555041
Show Author Affiliations
Alexey S. Nizamutdinov, Kazan Federal Univ. (Russian Federation)
Taisia A. Telegina, Kazan Federal Univ. (Russian Federation)
Bach Institute of Biochemistry (Russian Federation)
Andrey A. Buglak, Saint Petersburg State Univ. (Russian Federation)
Elena V. Lukinova, Kazan Federal Univ. (Russian Federation)
Eduard I. Madirov, Kazan Federal Univ. (Russian Federation)
Yuliya L. Vechtomova, Kazan Federal Univ. (Russian Federation)
Bach Institute of Biochemistry (Russian Federation)
Mikhail S. Kritsky, Bach Institute of Biochemistry (Russian Federation)
Vadim V. Semashko, Kazan Federal Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 11322:
XIV International Conference on Pulsed Lasers and Laser Applications
Victor F. Tarasenko; Anton V. Klimkin; Maxim V. Trigub, Editor(s)

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