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Proceedings Paper

Quantitative phase microscopy based on color-multiplexed single-shot Fourier ptychography
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Paper Abstract

We present a single-shot quantitative phase imaging (QPI) method based on color-multiplexed Fourier ptychographic microscopy (FPM). Three light-emitting diode (LED) elements with respective R/G/B channels in a programmable LED array illuminate the specimen simultaneously, providing triangle oblique illuminations matching the numerical aperture (NA) of the objective lens precisely. A single color image sensor records the light transmitted through the specimen, and three monochromatic intensity images at each color channel are then separated and utilized to recover the phase of the specimen. After one-step deconvolution based on the phase contrast transfer function, the obtained initial phase map is further refined by the FPM-based iterative recovery algorithm to overcome pixel-aliasing and improve the phase recovery accuracy. The high-speed, high- throughput QPI capabilities of the proposed approach are demonstrated by achieving a half-pitch resolution of 345 nm across a wide FOV of 1.33 mm2 at camera-limited frame rates (50 fps).

Paper Details

Date Published: 19 November 2019
PDF: 6 pages
Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 111861K (19 November 2019); doi: 10.1117/12.2554998
Show Author Affiliations
Jiasong Sun, Nanjing Univ. of Science and Technology (China)
Jiangsu Key Lab. of Spectral Imaging & Intelligent Sense (China)
Smart Computational Imaging Lab. (China)
Chao Zuo, Nanjing Univ. of Science and Technology (China)
Jiangsu Key Lab. of SPectral Imaging & Intelligent Sense (China)
Smart Computational Imaging Lab. (China)
Qian Chen, Nanjing Univ. of Science and Technology (China)

Published in SPIE Proceedings Vol. 11186:
Advanced Optical Imaging Technologies II
Xiao-Cong Yuan; P. Scott Carney; Kebin Shi; Michael G. Somekh, Editor(s)

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