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Proceedings Paper

Large-scale polarization contrast optical diffraction tomography (Conference Presentation)
Author(s): Jeroen Kalkman; Jos van Rooij

Paper Abstract

We demonstrate large scale high sensitivity optical diffraction tomography (ODT) imaging of zebrafish. We make this possible by three improvements. First, we optimize the field of view by using a high magnification over numerical aperture ratio ODT set-up with phase stepping. Second, we decrease the noise in the reconstructed images by off-axis sample placement, numerical focus tracking, and acquisition of a large number of projections. Third, we optimize the tissue clearing procedure to prevent scattering and refraction. We demonstrate our technique by imaging a zebrafish over a 4.1x4.1x5.5 mm3 volume with 4 micrometer spatial resolution. In addition, we demonstrate with the same set-up combined phase and polarization contrast optical diffraction tomography imaging. We use the phase and amplitude of the digital hologram to reconstruct the refractive index and (scaled) birefringence, respectively. Birefringence contrast imaging is demonstrated on zebrafish and shows high contrast images of the muscle tissue, something that is not well visible in conventional phase-based optical diffraction tomography.

Paper Details

Date Published: 1 April 2020
Proc. SPIE 11351, Unconventional Optical Imaging II, 1135105 (1 April 2020); doi: 10.1117/12.2554560
Show Author Affiliations
Jeroen Kalkman, Technische Univ. Delft (Netherlands)
Jos van Rooij, Technische Univ. Delft (Netherlands)

Published in SPIE Proceedings Vol. 11351:
Unconventional Optical Imaging II
Corinne Fournier; Marc P. Georges; Gabriel Popescu, Editor(s)

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