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Proceedings Paper

A diode laser based clinical diagnostic system using shifted excitation resonance Raman difference spectroscopy for the in vivo detection of ß-carotene in human skin
Author(s): Bernd Sumpf; Marcel Braune; Martin Maiwald; Maxim E. Darvin; Jürgen Lademann; Günther Tränkle
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Paper Abstract

Non-invasive in vivo monitoring of the antioxidant level of human skin, e.g. by measuring carotenoid marker substances, can deliver significant data on human health status and can provide diagnostic data during medical treatments. Among others, resonance Raman spectroscopy is a promising contactless tool to detect carotenoids in human skin within the necessary nanomolar concentration range. Unfortunately, laser induced fluorescence and ambient light can obscure the Raman signals even when using the resonance Raman effect and exciting the carotenoids at wavelengths around 500 nm. Here, shifted excitation resonance Raman difference spectroscopy (SERRDS) is a powerful and easyto- use tool separating the wanted from the unwanted signals. For this purpose, a portable clinical diagnostic system including a compact Raman handheld probe and a miniaturized wavelength-tunable frequency-doubled diode laserbased 488 nm light source was developed. The diode laser can be tuned over 2 nm providing the two excitation lines with a flexible spectral distance for SERRDS, resulting fluorescence-free skin Raman spectra. For reliable and representative measurements of human skin, an excitation spot diameter of 3 mm was selected. An excitation power of 9 mW at the sample provides a power density of 1.3 mW/mm2 meeting the laser safety regulations with a maximum permissible exposure of 20 mW/mm2. A calibration procedure was performed using skin phantoms containing ßcarotene at selected concentrations and a limit of detection of 0.05 nmol g-1 of β-carotene is achieved using the 3-sigma criterion.

Paper Details

Date Published: 3 March 2020
PDF: 8 pages
Proc. SPIE 11257, Plasmonics in Biology and Medicine XVII, 1125707 (3 March 2020); doi: 10.1117/12.2554015
Show Author Affiliations
Bernd Sumpf, Ferdinand-Braun-Institut (Germany)
Marcel Braune, Ferdinand-Braun-Institut (Germany)
Martin Maiwald, Ferdinand-Braun-Institut (Germany)
Maxim E. Darvin, Charité Universitätsmedizin Berlin (Germany)
Jürgen Lademann, Charité Universitätsmedizin Berlin (Germany)
Günther Tränkle, Ferdinand-Braun-Institut (Germany)
Technische Univ. Berlin (Germany)

Published in SPIE Proceedings Vol. 11257:
Plasmonics in Biology and Medicine XVII
Tuan Vo-Dinh; Ho-Pui A. Ho; Krishanu Ray, Editor(s)

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