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Proceedings Paper

MINFLUX: Achieving the ultimate resolution limit in fluorescence microscopy (Conference Presentation)
Author(s): Francisco Balzarotti; Jasmin K. Pape; Klaus C. Gwosch; Stefan W Hell

Paper Abstract

Superresolution microscopy methods have revolutionized far-field optical fluorescence microscopy by manipulating state transitions of the emitters, offering potentially unlimited resolution. In practice, however, the resolution of an image is limited by the finite photon budget of fluorescent probes. The recently introduced localization concept, termed MINFLUX, tackles this limitation by rendering each emitted photon more informative, achieving single digit nanometer resolution. Here, we present a MINFLUX strategy with high photon efficiency in arbitrarily large regions that allows imaging in fixed and living cells. This allows isotropic localization precision and surpasses the typical ∝1⁄√N dependence. A multi-color modality for 3D-MINFLUX imaging will be also presented, together with several biological applications.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460S (9 March 2020); doi: 10.1117/12.2553661
Show Author Affiliations
Francisco Balzarotti, Research Institute of Molecular Pathology (Austria)
Jasmin K. Pape, Max-Planck-Institut für Biophysikalische Chemie (Germany)
Klaus C. Gwosch, Max-Planck-Institut für Biophysikalische Chemie (Germany)
Stefan W Hell, Max-Planck-Institut für Biophysikalische Chemie (Germany)

Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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