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Proceedings Paper

From multi-photon absorption and stimulated emission to forbidden S0→T1 transition (Conference Presentation)

Paper Abstract

We will overview our initial developments of two, three, and four-photon excitation that started in early nineties with parallel developments in two-color two photon excitation to applications of light quenching (light stimulated emission) as a process to shape the excited state population. We will summarize our basic spectroscopy and time-resolved work in a cuvette system to total internal reflection applications. We will present more recent application of multi-photon processes in the case of metal enhanced fluorescence (MAF) and surface plasmons coupled emission (SPCE). Finally we will present the most recent observation of directly excited phosphorescence (S0→T1 excitation) and discuss potential applications in protein studies and cellular imaging.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441I (9 March 2020); doi: 10.1117/12.2551828
Show Author Affiliations
Zygmunt K. Gryczynski, Texas Christian Univ. (United States)
Ignacy Gryczynski, Univ. of North Texas Health Science Ctr. at Fort Worth (United States)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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