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Proceedings Paper

3,3’-thiodipropanol as a refractive index matching mounting medium for fluorescence microscopy
Author(s): Milvia Iris Alata Tejedo; Juan Carlos Martinez Cervantes; Valeria Piazza
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Paper Abstract

In this work, several tests of the performance of 3,3’-thiodipropanol (TDP) as a mounting medium for fluorescence microscopy of biological samples were performed. Besides optical properties like the dispersion curve of TDP and the effect of the embedding medium on the fluorescence of commonly used dyes, the interaction with biological specimens, including the labeling of filamentous actin with fluorescent phalloidins, was tested: TDP showed to represent an interesting alternative to commercial mounting media. By mixing TDP with 2,2’-thiodiethanol (TDE), it was possible not only to fine tune the refractive index of the resulting solution, but to preserve the compatibility with fluorescent phalloidins.

Paper Details

Date Published: 17 February 2020
PDF: 5 pages
Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 1124514 (17 February 2020); doi: 10.1117/12.2551802
Show Author Affiliations
Milvia Iris Alata Tejedo, Ctr. de Investigaciones en Óptica, A.C. (Mexico)
Juan Carlos Martinez Cervantes, Ctr. de Investigaciones en Óptica, A.C. (Mexico)
Valeria Piazza, Ctr. de Investigaciones en Óptica, A.C. (Mexico)


Published in SPIE Proceedings Vol. 11245:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Thomas G. Brown; Tony Wilson; Laura Waller, Editor(s)

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