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Proceedings Paper

Repurposing protein degradation for optogenetic modulation of protein activities (Conference Presentation)
Author(s): Payel Mondal

Paper Abstract

Non-neuronal optogenetic approaches empower precise regulation of protein dynamics in live cells but often require target-specific protein engineering. To address this challenge, we developed a generalizable light modulated protein stabilization system (GLIMPSe) to control intracellular protein level independent of its functionality. We applied GLIMPSe to control two distinct classes of proteins, phosphatases and kinases. Kinetics study showed that light-induced protein stabilization could be achieved within 30 minutes of blue light stimulation. Overall, GLIMPSe enables target-independent optogenetic control of protein activities, minimizes the systematic variation embedded within different photoactivatable proteins and promises to achieve light-mediated post-translational stabilization of a wide array of target proteins in live cells.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11227, Optogenetics and Optical Manipulation 2020, 112270E (9 March 2020); doi: 10.1117/12.2551652
Show Author Affiliations
Payel Mondal, Univ. of Illinois (United States)

Published in SPIE Proceedings Vol. 11227:
Optogenetics and Optical Manipulation 2020
Samarendra K. Mohanty; E. Duco Jansen; Anna W. Roe, Editor(s)

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