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Proceedings Paper

Optimization of optical clearing and large-scale 3D imaging of whole intact murine lung (Conference Presentation)
Author(s): Lorenzo F. Ochoa

Paper Abstract

Numerous pulmonary disorders are associated with fibrosis, however, current methodology falls short when studying turbid tissue. Optical clearing (OC) offers a chemical-based approach for deep tissue imaging. We optimized organ-level OC approach and combined it with large-scale, label-free multiphoton microscopy (MPM) and second harmonic generation microscopy (SHGM) to reveal fibrillar collagen in whole murine lungs. The standardization of several underlying steps allowed for a streamline approach. This method revealed significant differences in collagen deposition between control and treated lungs, while establishing a new approach based on OC and MPM/SHGM imaging for 3D analysis of lung fibrosis in the whole lung.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11231, Design and Quality for Biomedical Technologies XIII, 1123109 (9 March 2020);
Show Author Affiliations
Lorenzo F. Ochoa, The Univ. of Texas Medical Branch (United States)

Published in SPIE Proceedings Vol. 11231:
Design and Quality for Biomedical Technologies XIII
Jeeseong Hwang; Gracie Vargas, Editor(s)

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