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Proceedings Paper

Volumetric imaging of synaptic activity at depth by adaptive optical Bessel focus scanning multiphoton microscopy (Conference Presentation)
Author(s): Wei Chen; Ryan Natan; Yuhan Yang; Na Ji

Paper Abstract

An enabling technology for the monitoring of neural activity, multiphoton microscopy with Bessel focus scanning is a high-speed volumetric imaging method with subcellular lateral resolution. Similar to many other optical microscopy techniques, however, its axially extended Bessel-like focus experiences sample-induced optical aberrations, which lead to reduced resolution and image contrast at depth. In this study, we demonstrated an adaptive optical Bessel focus scanning multiphoton microscope with pupil-segmentation-based wavefront sensing and highly efficient sample-plane aberration correction. Applying it to mouse brain imaging in vivo, we observed up to threefold signal enhancement for functional spine imaging at depth.

Paper Details

Date Published: 11 March 2020
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Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 1124803 (11 March 2020);
Show Author Affiliations
Wei Chen, Univ. of California, Berkeley (United States)
Ryan Natan, Univ. of California, Berkeley (United States)
Yuhan Yang, Univ. of California, Berkeley (United States)
Na Ji, Univ. of California, Berkeley (United States)
Lawrence Berkeley National Lab. (United States)


Published in SPIE Proceedings Vol. 11248:
Adaptive Optics and Wavefront Control for Biological Systems VI
Thomas G. Bifano; Sylvain Gigan; Na Ji, Editor(s)

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