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Proceedings Paper

Exploring the spatial precision of focal infrared neural stimulation in the cortex of GCaMP6f mice
Author(s): Attila Kaszas; Ivo Vanzetta; Gergely Szalay; Alexandra Bojdán; Balázs Rózsa; Rodney O'Connor; David Moreau
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Paper Abstract

Functional mapping of the connectivity of the brain is an essential procedure for neurosurgeons aiming for maximal resection of a brain tumor, while minimizing damage to the eloquent cortex. Being able to delineate the location of critical cortical areas in individual patients allows the surgeon to preserve sensorimotor and cognitive functions. Several methods exist for functional mapping, however, none are capable of delivering fast, label free mapping of the cortex with millimeter precision. In this work, we investigate the potential use of infrared neural stimulation (λ=1470nm) as a means of focal stimulation of the mouse cortex in vivo. Here, we show that the application of infrared neural stimulation induces in vivo cortical intracellular calcium signals in Layer II/III mouse neurons, using intravital calcium imaging with the genetically encoded calcium indicator GCaMP6f.

Paper Details

Date Published: 19 February 2020
PDF: 6 pages
Proc. SPIE 11227, Optogenetics and Optical Manipulation 2020, 112270T (19 February 2020);
Show Author Affiliations
Attila Kaszas, Institut de Neurosciences de la Timone (France)
Aix-Marseille Univ. (France)
Ivo Vanzetta, Institut de Neurosciences de la Timone (France)
Aix Marseille Univ. (France)
Gergely Szalay, Institute of Experimental Medicine (Hungary)
Alexandra Bojdán, Institute of Experimental Medicine (Hungary)
Balázs Rózsa, Institute of Experimental Medicine (Hungary)
Pázmány Péter Catholic Univ. (Hungary)
Rodney O'Connor, Institut de Neurosciences de la Timone (France)
MINES Saint-Étienne (France)
David Moreau, MINES Saint-Étienne (France)

Published in SPIE Proceedings Vol. 11227:
Optogenetics and Optical Manipulation 2020
Samarendra K. Mohanty; E. Duco Jansen; Anna W. Roe, Editor(s)

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