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Proceedings Paper

Wide-field label-free imaging of thick tissues using optical diffraction tomography (Conference Presentation)
Author(s): Herve Hugonnet; Moosung Lee; Seungwoo Shin; Yongkeun Park

Paper Abstract

Optical diffraction tomography (ODT) is a label-free three-dimensional (3D) microscopy technique allowing to retrieve the refractive-index distributions of optically translucent samples. Until recently, the histopathological applications of ODT have been impeded by the difficulty of imaging thick and wide tissues. Here, we demonstrate rapid, wide-field 3D ODT for imaging biological tissues. We designed a high-speed stitching ODT platform exploiting a digital micromirror device and a motorized stage. Developing a fast algorithmic framework that relieves the stitching artifacts and contrast loss due to multiple scattering, we demonstrate high-contrast 3D imaging of 100-m-thick, square-millimeter-wide pathological tissues at sub-micrometer resolution.

Paper Details

Date Published: 11 March 2020
Proc. SPIE 11249, Quantitative Phase Imaging VI, 1124911 (11 March 2020); doi: 10.1117/12.2551117
Show Author Affiliations
Herve Hugonnet, KAIST (Korea, Republic of)
Moosung Lee, KAIST (Korea, Republic of)
Seungwoo Shin, KAIST (Korea, Republic of)
Yongkeun Park, KAIST (Korea, Republic of)

Published in SPIE Proceedings Vol. 11249:
Quantitative Phase Imaging VI
Yang Liu; Gabriel Popescu; YongKeun Park, Editor(s)

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