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Proceedings Paper

25 plane multifocus microscopy for fast and live 3D imaging (Conference Presentation)

Paper Abstract

We here report an optical system for live 3D microscopy that we call the Multifocus 25-camera microscope (M25). M25 is a new design for aberration-corrected multifocus microscopy (MFM) that employs the latest generation of small, fast and sensitive CMOS cameras. Each of the 25 focal planes is captured on one of the individual cameras, enabling truly simultaneous 3D recording at >100Hz. M25 is built from a combination of custom manufactured diffractive Fourier optics elements and off-the-shelf components. We are employing M25 to study neural circuit function in small model organisms including C. elegans, Drosophila, and fish.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11226, Neural Imaging and Sensing 2020, 1122602 (9 March 2020); doi: 10.1117/12.2550979
Show Author Affiliations
Eduardo Hirata Miyasaki, Univ. of California, Santa Cruz (United States)
Gustav M. Pettersson, KTH Royal Institute of Technology (Sweden)
Khant Zaw, Univ. of California, Santa Cruz (United States)
Demis D. John, Univ. of California, Santa Barbara (United States)
Brian Thibeault, Univ. of California, Santa Barbara (United States)
Brandon Lynch, Univ. of California, Santa Cruz (United States)
Juliana Hernandez, Univ. of California, Santa Cruz (United States)
Sara Abrahamsson, Univ. of California, Santa Cruz (United States)


Published in SPIE Proceedings Vol. 11226:
Neural Imaging and Sensing 2020
Qingming Luo; Jun Ding; Ling Fu, Editor(s)

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