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Proceedings Paper

Two-photon phosphorescence lifetime microscopy (2PLM) of Oxygen (Conference Presentation)
Author(s): Sergei A. Vinogradov

Paper Abstract

Molecular oxygen plays a unique role in cellular energy metabolism by serving as the terminal electron acceptor in the mitochondrial respiratory chain. Quantitative imaging of oxygen can provide invaluable information about metabolism in normal and diseased states. Two-photon phosphorescence lifetime microscopy (2PLM) enables imaging of oxygen concentration gradients in 3D with micron-scale resolution. The method has found use in neuroscience, stem cell and tumor biology. We will discuss the principles of 2PLM and focus on the new phosphorescent probes, which enable imaging of oxygen simultaneously with imaging of temperature.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124416 (9 March 2020); doi: 10.1117/12.2550690
Show Author Affiliations
Sergei A. Vinogradov, Univ. of Pennsylvania (United States)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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