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Proceedings Paper

Towards easier, faster, super-resolved microscopy
Author(s): Frederik Görlitz; Riccardo Wysoczanski; Sunil Kumar; Jonathan Lightley; Edwin Garcia; Yuriy Alexandrov; Ian Munro; Simon Johnson; Martin Kehoe; Callum Hollick; Jeremy Graham; Louise Donnelly; Peter Barnes; Christopher Dunsby; Mark A. A. Neil; Paul M. W. French
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Paper Abstract

Super-resolved microscopy techniques have overcome the diffraction limit to provide image resolutions approaching the scale of fluorescent labels. However, many of these techniques require significant experimental resources and expertise and impose long image data acquisition times, making it difficult to acquire super-resolved data from sufficiently large sample numbers to overcome intrinsic biological variation. We have worked to make stimulated emission depletion (STED) microscopy and single molecule localisation microscopy (SMLM) more straightforward to implement and more practical to image larger numbers of cells. Here we present work in progress developing easySLM STED and easySTORM, including a new modular microscope frame that we believe can make it easier to prototype microscopy techniques and to implement and maintain them in lower resourced settings.

Paper Details

Date Published: 3 March 2020
PDF: 7 pages
Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460R (3 March 2020); doi: 10.1117/12.2550682
Show Author Affiliations
Frederik Görlitz, Imperial College London (United Kingdom)
Riccardo Wysoczanski, Imperial College London (United Kingdom)
Sunil Kumar, Imperial College London (United Kingdom)
The Francis Crick Institute (United Kingdom)
Jonathan Lightley, Imperial College London (United Kingdom)
Edwin Garcia, Imperial College London (United Kingdom)
Yuriy Alexandrov, Imperial College London (United Kingdom)
The Francis Crick Institute (United Kingdom)
Ian Munro, Imperial College London (United Kingdom)
Simon Johnson, Imperial College London (United Kingdom)
Martin Kehoe, Imperial College London (United Kingdom)
Callum Hollick, Cairn Research Ltd. (United Kingdom)
Jeremy Graham, Cairn Research Ltd. (United Kingdom)
Louise Donnelly, Imperial College London (United Kingdom)
Peter Barnes, Imperial College London (United Kingdom)
Christopher Dunsby, Imperial College London (United Kingdom)
Mark A. A. Neil, Imperial College London (United Kingdom)
Paul M. W. French, Imperial College London (United Kingdom)


Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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