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Proceedings Paper

Observations of the bowel wall in the case of acute ischemia: optical coherence tomography, FLIM macro-imaging and histological analysis data
Author(s): Elena B. Kiseleva; Maxim Ryabkov; Evgenia L. Bederina; Marina V. Shirmanova; Mikhail S. Baleev; Maria M. Lukina; Marina A. Sirotkina; Aleksandra S. Chilipenok; Vladimir V. Beschastnov; Alexander A. Moiseev; Elena V. Zagaynova; Natalia D. Gladkova
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Paper Abstract

The objective of the research was a multifaceted study of strangulated small intestine to reveal the optical, morphofunctional and biochemical signs of small bowel ischemia. The study was carried out in vivo using an artificially induced strangulation model of the small intestine (together with its mesentery and blood vessels) in 12 Wistar rats. Over a period of 120 minutes following the bowel ligation, changes in the density of the intramural vasculature and intestinal wall microstructure were detected using multimodal optical coherence tomography (MM OCT). Fluorescence lifetime changes of endogenous fluorophores were also measured using macro-FLIM of the strangulated loop and the adjacent segments of the intestine. At the end of the experiment, a morphometric study of the thickness of the layers and the prevalence of necrosis in the intestinal wall was carried out. A comprehensive analysis of the results of the OCT, FLIM and morphometry of the ischemic wall of the small intestine made it possible to determine the correlating morphofunctional and biochemical manifestations that are specific to this model of mesenteric blood flow disturbance.

Paper Details

Date Published: 17 February 2020
PDF: 5 pages
Proc. SPIE 11232, Multimodal Biomedical Imaging XV, 112320M (17 February 2020); doi: 10.1117/12.2550667
Show Author Affiliations
Elena B. Kiseleva, Privolzhsky Research Medical Univ. (Russian Federation)
Maxim Ryabkov, Privolzhsky Research Medical Univ. (Russian Federation)
City Clinical Hospital No. 30 (Russian Federation)
Evgenia L. Bederina, Privolzhsky Research Medical Univ. (Russian Federation)
Marina V. Shirmanova, Privolzhsky Research Medical Univ. (Russian Federation)
Mikhail S. Baleev, City Clinical Hospital No. 30 (Russian Federation)
Maria M. Lukina, Privolzhsky Research Medical Univ. (Russian Federation)
Marina A. Sirotkina, Privolzhsky Research Medical Univ. (Russian Federation)
Aleksandra S. Chilipenok, Privolzhsky Research Medical Univ. (Russian Federation)
Vladimir V. Beschastnov, City Clinical Hospital No. 30 (Russian Federation)
Alexander A. Moiseev, Institute of Applied Physics (Russian Federation)
Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation)
Natalia D. Gladkova, Privolzhsky Research Medical Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 11232:
Multimodal Biomedical Imaging XV
Fred S. Azar; Xavier Intes; Qianqian Fang, Editor(s)

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