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Proceedings Paper

Ultrafast fluorescence lifetime imaging microscopy by frequency-division multiplexing (Conference Presentation)

Paper Abstract

We demonstrate ultrafast fluorescence lifetime imaging microscopy (FLIM) based on frequency-division multiplexing. As a proof-of-concept demonstration, we obtained images with fluorescence intensity and lifetime contrasts of MCF-7 breast cancer cells stained by SYTO16 at a record high frame rate of 16,000 fps, which is 100 times higher than that of previously reported FLIM techniques. Our method is expected to expand the utility of FLIM to quantitative analysis of rapid intracellular dynamics and high-throughput cell screening based on fluorescence lifetime images.

Paper Details

Date Published: 26 March 2020
Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460D (26 March 2020); doi: 10.1117/12.2550427
Show Author Affiliations
Hiroshi Kanno, The Univ. of Tokyo (Japan)
Hideharu Mikami, The Univ. of Tokyo (Japan)
Japan Science and Technology Agency (Japan)
Keisuke Goda, The Univ. of Tokyo (Japan)
Univ. of California (United States)
Wuhan Univ. (China)

Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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