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Proceedings Paper

Confocal Raman microspectroscopy for evaluation of optical clearing efficiency of the skin ex vivo
Author(s): I. Yu. Yanina; J. Schleusener; J. Lademann; V. V. Tuchin; M. E. Darvin
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Paper Abstract

Optical clearing (OC) of biological tissues is a promising technology for a wide spread use in medical practice to increase the screening depth, spatial resolution and contrast of the resulting images/spectra. Nevertheless, despite the significant OC effect, some biocompatible optical clearing agents (OCAs) can adversely affect biological tissues, causing local hemostasis, morphological changes, d ehydration, and in some cases even tissue necrosis. The aim of this study was to study the effect of Omnipaque 300 and fructose solutions of various concentrations and exposure times on the intact skin using confocal Raman microspectroscopy. It was shown that the application of each of these OCAs on intact skin for 5 min also leads to an appreciable OC effect. The increase in OC was achieved using a mixture of Omnipaque 300 with DMSO; it was shown that the optical properties of the skin can be controlled at a depth of about 80 μm.

Paper Details

Date Published: 21 February 2020
PDF: 7 pages
Proc. SPIE 11239, Dynamics and Fluctuations in Biomedical Photonics XVII, 112390W (21 February 2020); doi: 10.1117/12.2550352
Show Author Affiliations
I. Yu. Yanina, Saratov State Univ. (Russian Federation)
Tomsk State Univ. (Russian Federation)
Universitätsmedizin Berlin (Germany)
J. Schleusener, Charité Universitätsmedizin Berlin (Germany)
J. Lademann, Charité Universitätsmedizin Berlin (Germany)
V. V. Tuchin, Saratov State Univ. (Russian Federation)
Tomsk State Univ. (Russian Federation)
Institute of Precision Mechanics and Control (Russian Federation)
M. E. Darvin, Charité Universitätsmedizin Berlin (Germany)


Published in SPIE Proceedings Vol. 11239:
Dynamics and Fluctuations in Biomedical Photonics XVII
Valery V. Tuchin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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