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Proceedings Paper

Quantifying myelination of single neurons using Spatial Light Interference Microscopy (SLIM) (Conference Presentation)
Author(s): Michael J. Fanous; Young Jae Lee; Catherine A. Best-Popescu; Allison Louie; Andrew Steelman; Megan P. Caputo; Laurie A. Rund; Rodney W. Johnson; Tapas Das; Matthew J. Kuchan; Gabriel Popescu

Paper Abstract

Although both neurons and oligodendrocytes have been well studied individually, very little is known about how they interact with each other. New methods are needed to further study the intricacies of this interplay in terms of cellular and molecular dynamics. Spatial Light Interference Microscopy (SLIM) is a quantitative phase imaging technique that generates phase maps related to the dry mass content of the sample. In this work, we study the ability of SLIM to quantify myelination at the axonal level. We imaged a series of cocultures comprising hippocampal neurons and oligodendrocytes, of varying densities, using SLIM, and evaluated dry mass formation and growth of myelin.

Paper Details

Date Published: 11 March 2020
Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490T (11 March 2020); doi: 10.1117/12.2550330
Show Author Affiliations
Michael J. Fanous, Beckman Institute for Advanced Science and Technology (United States)
Young Jae Lee, Beckman Institute for Advanced Science and Technology (United States)
Catherine A. Best-Popescu, Beckman Institute for Advanced Science and Technology (United States)
Allison Louie, Univ. of Illinois (United States)
Andrew Steelman, Univ. of Illinois (United States)
Megan P. Caputo, Univ. of Illinois (United States)
Laurie A. Rund, Univ. of Illinois (United States)
Rodney W. Johnson, Univ. of Illinois (United States)
Tapas Das, Abbott Nutrition (United States)
Matthew J. Kuchan, Abbott Nutrition (United States)
Gabriel Popescu, Beckman Institute for Advanced Science &and Technology (United States)

Published in SPIE Proceedings Vol. 11249:
Quantitative Phase Imaging VI
Yang Liu; Gabriel Popescu; YongKeun Park, Editor(s)

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