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Proceedings Paper

A multiplexed confocal FLIM microscope with 4-taps time-gated imager
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Paper Abstract

Combined with confocal imaging, Fluorescence lifetime imaging microscopy (FLIM) can achieve 3-dimensional optical sectional capability with sub-nanosecond lifetime information. As confocal FLIM acquires multi-dimensional data 4D (3D space + time), it is inherently slow. Recent developments in lock-in pixel imagers with time gated pixels show such detectors are capable of collecting as many as 8-time gates in a single pixel cycle. We present a multiplexed confocal FLIM microscope, equipped with a 4-taps time-gated lock-in pixel imager. The multiplexing setup allows the use of the sparse array with sub-nanosecond time-gating to achieve high throughput FLIM acquisition.

Paper Details

Date Published: 17 February 2020
PDF: 5 pages
Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 1124512 (17 February 2020); doi: 10.1117/12.2550208
Show Author Affiliations
Morgan Richards, McMaster Univ. (Canada)
Yuya Shirakawa, Shizuoka Univ. (Japan)
Fares Badr, McMaster Univ. (Canada)
Keiichiro Kagawa, Shizuoka Univ. (Japan)
Shoji Kawahito, Shizuoka Univ. (Japan)
Qiyin Fang, McMaster Univ. (Canada)

Published in SPIE Proceedings Vol. 11245:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Thomas G. Brown; Tony Wilson; Laura Waller, Editor(s)

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