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Proceedings Paper

Combined scattering and fluorescent measurement of the retina in vivo reveals disease-dependent changes in intrinsic fluorophores of retinal pigment epithelium (Conference Presentation)

Paper Abstract

A combined scattering and autofluorescence (AF) measurement from the retinal pigment epithelium (RPE) was investigated to study the changes in the AF granules in an Abca4-/- mouse (murine model of Stargardt disease). The directional scattering was measured with an optical coherence tomography (OCT), whereas the multi-color fundus AF spectra was measured with a spectrometer-integrated scanning laser ophthalmoscope. Increased scattering from the RPE in Abca4-/- mouse relative to control was well correlated with the elevated AF spectra and indicated the changes in RPE fluorophores. Ex vivo studies based on scanning confocal and electron microscopy were performed to validate the in vivo findings.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11218, Ophthalmic Technologies XXX, 112181D (9 March 2020); doi: 10.1117/12.2550163
Show Author Affiliations
Ratheesh Kumar Meleppat, Univ. of California, Davis (United States)
Gabriel Peinado, Univ. of California, Davis (United States)
Kaitryn E. Ronning, Univ. of California, Davis (United States)
Sarah J. Karlen, Univ. of California, Davis (United States)
Pengfei Zhang, Univ. of California, Davis (United States)
Edward N. Pugh Jr., Univ. of California, Davis (United States)
Robert J. Zawadzki, Univ. of California, Davis (United States)


Published in SPIE Proceedings Vol. 11218:
Ophthalmic Technologies XXX
Fabrice Manns; Arthur Ho; Per G. Söderberg, Editor(s)

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