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Proceedings Paper

Imaging plasma membrane microviscosity in cancer cells during chemotherapy
Author(s): Liubov E. Shimolina; Marina V. Shirmanova; Marina K. Kuimova; Maria M. Lukina; Nadezhda I. Ignatova; Irina N. Druzhkova; Elena V. Zagaynova
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Paper Abstract

Recent studies suggest that cancer cell response to cisplatin can not be fully described in terms of only interaction of the drug with DNA, but can include effects associated with other cellular targets. The study of effects of chemotherapeutic drugs on the viscosity of plasma membrane is important for better understanding the mechanisms of the drug action and evaluating the effectiveness of therapy. The aim of this work was to analyze microviscosity of plasma membrane of cancer cells during chemotherapy with cisplatin. For imaging viscosity at the microscopic level fluorescent molecular rotor BODIPY2 and fluorescence lifetime imaging microscopy (FLIM) were used. We detected a significant increase in membrane viscosity in viable human cervical cancer cells HeLa, both in cell monolayer and tumor spheroids after cisplatin treatment. Measuring viscosity in cisplatin-resistant cell line showed that viscosity increases when cells acquire chemoresistance. These results suggest that microviscosity of membrane plays a role in the cytotoxicity of cisplatin and its mapping may provide a powerful tool for investigation of tumor responses to chemotherapy and mechanisms of drug resistance.

Paper Details

Date Published: 14 February 2020
PDF: 7 pages
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112442M (14 February 2020); doi: 10.1117/12.2550133
Show Author Affiliations
Liubov E. Shimolina, Privolzhsky Research Medical Univ. (Russian Federation)
N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation)
Marina V. Shirmanova, Privolzhsky Research Medical Univ. (Russian Federation)
Marina K. Kuimova, Imperial College London (United Kingdom)
Maria M. Lukina, Privolzhsky Research Medical Univ. (Russian Federation)
Nadezhda I. Ignatova, Privolzhsky Research Medical Univ. (Russian Federation)
Irina N. Druzhkova, Privolzhsky Research Medical Univ. (Russian Federation)
Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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