Share Email Print
cover

Proceedings Paper

Free-electron-mediated modifications of biomolecules: from photodamage in nonlinear microscopy to intentional photomodification of cells and tissues (Conference Presentation)

Paper Abstract

Femtosecond laser-induced plasma generation is used surgically and may also cause photodamage in nonlinear microscopy. Photodamage in multiphoton microscopy already starts at irradiances 1.5 times above the value used for autofluorescence imaging but the cavitation bubble threshold is 20 x higher. We explore the realm of low-density plasma effects between multi-pulse nonlinear imaging and single-pulse surgical regime. We characterize the transition from unchanged tissue (emitting autofluorescence) to slightly changed tissue (hyperfluorescence), drastically changed tissue (plasma luminescence) and disintegrated biomolecules (gas bubble formation). By plotting the threshold values in (irradiance, radiant exposure) space, we identified a “safe” region for nonlinear microscopy.

Paper Details

Date Published: 9 March 2020
PDF
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112440I (9 March 2020); doi: 10.1117/12.2549643
Show Author Affiliations
Alfred Vogel, Univ. zu Lübeck (Germany)
Xiao-Xuan Liang, Univ. zu Lübeck (Germany)
Sebastian Freidank, Univ. zu Lübeck (Germany)
Norbert Linz, Univ. zu Lübeck (Germany)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

© SPIE. Terms of Use
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray