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Proceedings Paper

Neutrophil Extracellular Traps (NETs): an unexplored territory in renal pathobiology, a pilot computational study
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Paper Abstract

In the age of modern medicine and artificial intelligence, image analysis and machine learning have revolutionized diagnostic pathology, facilitating the development of computer aided diagnostics (CADs) which circumvent prevalent diagnostic challenges. Although CADs will expedite and improve the precision of clinical workflow, their prognostic potential, when paired with clinical outcome data, remains indeterminate. In high impact renal diseases, such as diabetic nephropathy and lupus nephritis (LN), progression often occurs rapidly and without immediate detection, due to the subtlety of structural changes in transient disease states. In such states, exploration of quantifiable image biomarkers, such as Neutrophil Extracellular Traps (NETs), may reveal alternative progression measures which correlate with clinical data. NETs have been implicated in LN as immunogenic cellular structures, whose occurrence and dysregulation results in excessive tissue damage and lesion manifestation. We propose that renal biopsy NET distribution will function as a discriminate, predictive biomarker in LN, and will supplement existing classification schemes. We have developed a computational pipeline for segmenting NET-like structures in LN biopsies. NET-like structures segmented from our biopsies warrant further study as they appear pathologically distinct, and resemble nonlytic, vital NETs. Examination of corresponding H&E regions predominantly placed NET-like structures in glomeruli, including globally and segmentally sclerosed glomeruli, and tubule lumina. Our work continues to explore NET-like structures in LN biopsies by: 1.) revising detection and analytical methods based on evolving NETs definitions, and 2.) cataloguing NET morphology in order to implement supervised classification of NET-like structures in histopathology images.

Paper Details

Date Published: 16 March 2020
PDF: 7 pages
Proc. SPIE 11320, Medical Imaging 2020: Digital Pathology, 1132012 (16 March 2020); doi: 10.1117/12.2549340
Show Author Affiliations
Briana A. Santo, The State Univ. of New York at Buffalo (United States)
Brahm H. Segal, Roswell Park Comprehensive Cancer Ctr. (United States)
John E. Tomaszewski, The State Univ. of New York at Buffalo (United States)
Imtiaz Mohammad, The State Univ. of New York at Buffalo (United States)
Amber M. Worral, The State Univ. of New York at Buffalo (United States)
Sanjay Jain, Washington Univ. School of Medicine (United States)
Michelle B. Visser, Univ. at Buffalo School of Dental Medicine (United States)
Pinaki Sarder, The State Univ. of New York at Buffalo (United States)

Published in SPIE Proceedings Vol. 11320:
Medical Imaging 2020: Digital Pathology
John E. Tomaszewski; Aaron D. Ward, Editor(s)

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