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Proceedings Paper

High-speed, large field-of-view and deep imaging with an adaptive excitation source
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Paper Abstract

Conventional multiphoton microscopy uses periodically pulsed sources as excitation and the sample is illuminated uniformly by the laser. While necessary for structural imaging, monitoring dynamic biological functions such as neuronal activity in the brain typically only requires imaging of the region of interest (ROI), e.g., the neurons. The adaptive excitation source enables imaging of the region of interest only. It reduces the requirement for the output power of the excitation source (by at least an order of magnitude) and simultaneously reduces the excitation power to the sample for obtaining the necessary information (e.g., neuronal activity). We demonstrate three-photon imaging of brain activity in awake transgenic mice (jRGECO1a), with highest speed (30 frames/s), large field-of-view (620x620 μm/512x512 pixels) and deep penetration (750 μm beneath the dura).

Paper Details

Date Published: 14 February 2020
PDF: 4 pages
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112442L (14 February 2020); doi: 10.1117/12.2549312
Show Author Affiliations
Bo Li, Cornell Univ. (United States)
Chunyan Wu, Cornell Univ. (United States)
Mengran Wang, Cornell Univ. (United States)
Kriti Charan, Cornell Univ. (United States)
Chris Xu, Cornell Univ. (United States)

Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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