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Proceedings Paper

Molecular oxygen mapping in biological samples by time-correlated single photon counting technique and Ir(III)-based complexes
Author(s): Marina V. Shirmanova; Vladislav I. Shcheslavskiy; Maria M. Lukina; Varvara V. Dudenkova; Ilya S. Kritchenkov; Anastasia I. Solomatina; Sergey P. Tunik
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Paper Abstract

Non-invasive optical techniques on the basis of quenching of phosphorescence by molecular oxygen represent a powerful tool to perform mapping of oxygen content in biological systems. The development of phosphorescent oxygensensitive probes suitable for biological applications, especially in vivo, remains a challenging task. In this paper we applied for biological investigations one novel and one previously published Ir(III)-based complexes. This complexes demonstrate low cytotoxicity at the concentrations ≤70 μM and good uptake by cultured cancer cells. Both compounds were primarily located within the cytoplasm. Using TCSPC-based phosphorescence lifetime macro-imaging in vivo, we found that IR-2 complex shows detectable phosphorescence in tumor tissue in mice upon local injection. These results illustrate the high potential of organometallic complexes under study for mapping of oxygen level in cells and tissues.

Paper Details

Date Published: 21 February 2020
PDF: 8 pages
Proc. SPIE 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 112340G (21 February 2020); doi: 10.1117/12.2549248
Show Author Affiliations
Marina V. Shirmanova, Privolzhsky Research Medical Univ. (Russian Federation)
Vladislav I. Shcheslavskiy, Becker & Hickl GmbH (Germany)
Maria M. Lukina, Privolzhsky Research Medical Univ. (Russian Federation)
Varvara V. Dudenkova, Privolzhsky Research Medical Univ. (Russian Federation)
Ilya S. Kritchenkov, St. Petersburg State Univ. (Russian Federation)
Anastasia I. Solomatina, St. Petersburg State Univ. (Russian Federation)
Sergey P. Tunik, St. Petersburg State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 11234:
Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos; Angela B. Seddon, Editor(s)

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