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Proceedings Paper

D-FFOCT as a tool to assess oxygenation induced changes in cellular metabolism (Conference Presentation)
Author(s): Siddharth Khare; Ravi Malpani; Hyesoo Lee; Viswanath Gorti; Kosar Khaksari; Jules Scholler; Emilie Benoit; Claude Boccara; Dan Sackett; Amir Gandjbakhche

Paper Abstract

Dynamic Full-Field Optical Coherence Tomography (D-FFOCT) is a high transverse resolution version of OCT that records signal at the output of the interferometer as a movie over a few seconds. Analyzing the temporal variation of the signals reveals intracellular contrast which gives D-FFOCT the capability of identifying dynamic metabolic changes. We use D-FFOCT to identify oxygen induced changes in cellular metabolism at ambient (22%) oxygenation and at hypoxia (1%). Signal strength, calculated as an integral of the raw FFT spectrum, is significantly higher for 1% samples compared to 22% indicating that D-FFOCT is sensitive to changes in cell metabolism.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, 112281R (9 March 2020); doi: 10.1117/12.2549096
Show Author Affiliations
Siddharth Khare, Eunice Kennedy Shriver National Institute of Child Health and Human Development (United States)
Ravi Malpani, Eunice Kennedy Shriver National Institute of Child Health and Human Development (United States)
Hyesoo Lee, Univ. of Maryland (United States)
Viswanath Gorti, Eunice Kennedy Shriver National Institute of Child Health and Human Development (United States)
Kosar Khaksari, Eunice Kennedy Shriver National Institute of Child Health and Human Development (United States)
Jules Scholler, Institut Langevin Ondes et Images (France)
Emilie Benoit, LLTech SAS (France)
Claude Boccara, Institut Langevin Ondes et Images (France)
Dan Sackett, Eunice Kennedy Shriver National Institute of Child Health and Human Development (United States)
Amir Gandjbakhche, Eunice Kennedy Shriver National Institute of Child Health and Human Development (United States)


Published in SPIE Proceedings Vol. 11228:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV
Joseph A. Izatt; James G. Fujimoto, Editor(s)

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