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Proceedings Paper

In vivo chemical alteration profiling Caenorhabditis elegans during development and aging by hyperspectral stimulated Raman scattering microscopy (Conference Presentation)
Author(s): Tao Chen; Dinghuan Deng; Ayse Sena Mutlu; Yong Yu; Meng C. Wang

Paper Abstract

We systematically investigate the changes of lipid and protein contents at all developmental stages of Caenorhabditis elegans, including L1 to L4 larvae, and adults, using the recently developed hyperspectral stimulated Raman scattering (hsSRS) microscopy. The gut granules in larvae, which are known as lysosome-like organelles, are specifically identified and the temporal change of these gut granules are tracked at different stages. By acquiring the spectral images of C. elegans at different ages and analyzing the lipid spectra change statistically with hsSRS, we will foster a better understanding of the physiological and pathophysiological processes in living organisms.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125206 (10 March 2020); doi: 10.1117/12.2549054
Show Author Affiliations
Tao Chen, Baylor College of Medicine (United States)
Dinghuan Deng, Baylor College of Medicine (United States)
Ayse Sena Mutlu, Baylor College of Medicine (United States)
Yong Yu, Baylor College of Medicine (United States)
Meng C. Wang, Baylor College of Medicine (United States)
Howard Hughes Medical Institute (United States)


Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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