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Proceedings Paper

Supervised machine learning for region assignment of zebrafish brain nuclei based on computational assessment of cell neighborhoods
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Paper Abstract

Histological studies provide cellular insights into tissue architecture and have been central to phenotyping and biological discovery. Synchrotron X-ray micro-tomography of tissue, or “X-ray histotomography”, yields three-dimensional reconstruction of fixed and stained specimens without sectioning. These reconstructions permit the computational creation of histology-like sections in any user-defined plane and slice thickness. Furthermore, they provide an exciting new basis for volumetric, computational histological phenotyping at cellular resolution. In this paper, we demonstrate the computational characterization of the zebrafish central nervous system imaged by Synchrotron X-ray micro-CT through the classification of small cellular neighborhood volumes centered at each detected nucleus in a 3D tomographic reconstruction. First, we propose a deep learning-based nucleus detector to detect nuclear centroids. We then develop, train, and test a Convolutional Neural Network architecture for automatic classification of brain nuclei using five different neighborhood sizes, which correspond to 8, 12, 16, 20 and 24 isotropic voxel dimensions respectively. We show that even with small cell neighborhoods, our proposed model is able to characterize brain nuclei into the major tissue regions with a Jaccard score of 74.29% and F1 score of 85.34%. Using our detector and classifier, we obtained very good results for fully segmenting major zebrafish brain regions in the 3D scan through patch wise labeling of cell neighborhoods.

Paper Details

Date Published: 28 February 2020
PDF: 9 pages
Proc. SPIE 11317, Medical Imaging 2020: Biomedical Applications in Molecular, Structural, and Functional Imaging, 113170T (28 February 2020); doi: 10.1117/12.2548896
Show Author Affiliations
Samarth Gupta, The Pennsylvania State Univ. (United States)
Yuan Xue, The Pennsylvania State Univ. (United States)
Yifu Ding, The Pennsylvania State Univ. (United States)
Daniel Vanselow, The Pennsylvania State Univ. (United States)
Maksim Yakovlev, The Pennsylvania State Univ. (United States)
Damian B. vam Rossum, The Pennsylvania State Univ. (United States)
Sharon X. Huang, The Pennsylvania State Univ. (United States)
Keith C. Cheng, The Pennsylvania State Univ. (United States)


Published in SPIE Proceedings Vol. 11317:
Medical Imaging 2020: Biomedical Applications in Molecular, Structural, and Functional Imaging
Andrzej Krol; Barjor S. Gimi, Editor(s)

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