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Proceedings Paper

Biomechanical analysis of the embryonic mouse heart by optogenetic control (Conference Presentation)
Author(s): Andrew L. Lopez III; Shang Wang; Irina V. Larina

Paper Abstract

The role of biomechanical signaling is well accepted as a modulator of cardiac cell behavior and a requirement for cardiac morphogenesis. However, the small, fragile nature of the embryonic heart makes it difficult to determine transient mechanical homeostasis during heart development and search for causal links between biomechanical forces and cardiac cell behavior in vivo. Our work focuses on characterizing the regulatory role of biomechanical signals to direct cardiac morphogenesis and cell behavior. Towards this end, we have successfully established cardiac optogenetics to control heartbeat frequency in the embryonic mouse heart for the first time. We will combine this approach with second harmonic generation, an unbiased imaging approach to detect collagen deposition. Using optogenetic cardiac pacing and second harmonic generation imaging, we will look at how changes in heart biomechanics are consequential in the deposition and organization of cardiac collagen.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11215, Diagnostic and Therapeutic Applications of Light in Cardiology 2020, 112150J (9 March 2020); doi: 10.1117/12.2548853
Show Author Affiliations
Andrew L. Lopez III, Baylor College of Medicine (United States)
Shang Wang, Baylor College of Medicine (United States)
Irina V. Larina, Baylor College of Medicine (United States)

Published in SPIE Proceedings Vol. 11215:
Diagnostic and Therapeutic Applications of Light in Cardiology 2020
Kenton W. Gregory M.D.; Laura Marcu, Editor(s)

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