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Proceedings Paper

Real-time cellular phase imaging using dual-view transport of intensity phase microscopy
Author(s): Shaowei Sun; Zhilong Jiang; Yan Kong; Xiaoliang He; Cheng Liu; Shouyu Wang
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Paper Abstract

Transport of intensity phase microscopy (TIPM) can obtain sample phase images with commercial microscope and simple procedures; however, it requires multi-focal images limiting its real-time detection capability. In order to achieve dynamics of the live cells, we adopted dual-view TIPM which simultaneously captures under- and over-focus images for phase retrieval. In dual-view TIPM, two identify CMOS cameras were first set on the binocular tube of the microscope with different focal position of 3 mm difference to simultaneously capture both the under- and over-focus images. Afterwards, scaling, rotation and shifting are all corrected to maintain the same fields of view of under- and over-focus images. Next, the cellular phase can be extracted by solving the Poisson equation. The dual-view TIPM was finally adopted in live cell imaging, proving the dual-view TIPM can obtain real-time quantitative cellular phase imaging.

Paper Details

Date Published: 20 December 2019
PDF: 5 pages
Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112093F (20 December 2019); doi: 10.1117/12.2548834
Show Author Affiliations
Shaowei Sun, Jiangnan Univ. (China)
Zhilong Jiang, Jiangnan Univ. (China)
Yan Kong, Jiangnan Univ. (China)
Xiaoliang He, Jiangnan Univ. (China)
Cheng Liu, Jiangnan Univ. (China)
Shouyu Wang, Jiangnan Univ. (China)

Published in SPIE Proceedings Vol. 11209:
Eleventh International Conference on Information Optics and Photonics (CIOP 2019)
Hannan Wang, Editor(s)

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