Share Email Print

Proceedings Paper

Indirect action of VUV radiation on micro-organisms
Author(s): Galina N. Zvereva; Irina Yu. Kirtsidely; Eduard M. Machs; Elizaveta Yu. Letova
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

The contribution of the indirect mechanism to the inactivation of microorganisms by long wavelength VUV radiation (λ>170 nm) was investigated in this work. For these purposes dry spores of microscopic fungi Rhodotorula colostri and Saccharomyces cerevisiae were irradiated by Xe excilamp (λ= 173 nm).To determine the contribution of indirect action to the microfungi inactivation, the survival probabilities of the samples grown on a nutrient medium containing antioxidant (I2) and without it were carried out. The results have shown, that the survival probabilities of the spores containing antioxidant increases by no less than 20-25% compared to control ones. The increase value depends on VUV irradiation dose and microorganism type. It should be emphasized, that due to the masking of microfungi survival by I2 bactericidal action, the obtained survival probability increase values should be considered as a lower estimate. Electrophoresis of microfungi DNA have shown, that VUV irradiation of spores containing antioxidants leads to less DNA destruction and heaver DNA fragments appearance. The latter may indicate a decrease of reactive oxygen species effect on DNA in the presence of antioxidants. Atomic force microscopy and IR spectroscopy haven’t shown influence of antioxidant on cell wall destruction. This can indicate, that in contrast to wet spores, in the case of VUV irradiation of dry ones, the indirect mechanism of cell wall destruction is absent or weakly expressed.

Paper Details

Date Published: 11 December 2019
PDF: 7 pages
Proc. SPIE 11322, XIV International Conference on Pulsed Lasers and Laser Applications, 113222M (11 December 2019); doi: 10.1117/12.2548657
Show Author Affiliations
Galina N. Zvereva, State Univ. of Civil Aviation (Russian Federation)
S. I. Vavilov State Optical Institute (Russian Federation)
Irina Yu. Kirtsidely, Komarov Botanical Institute (Russian Federation)
Eduard M. Machs, Komarov Botanical Institute (Russian Federation)
Elizaveta Yu. Letova, ITMO Univ. (Russian Federation)

Published in SPIE Proceedings Vol. 11322:
XIV International Conference on Pulsed Lasers and Laser Applications
Victor F. Tarasenko; Anton V. Klimkin; Maxim V. Trigub, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?