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Proceedings Paper

High-throughput vibrational flow cytometry and beyond (Conference Presentation)
Author(s): Keisuke Goda; Yasuyuki Ozeki; Kotaro Hiramatsu; Yuta Suzuki

Paper Abstract

We introduce two newly developed methods for high-throughput vibrational flow cytometry, namely Fourier-transform coherent anti-Stokes Raman scattering flow cytometry and stimulated Raman scattering imaging flow cytometry, which cover the fingerprint and high-frequency regions with high throughputs of >1,000 cells/s and >100 cells/s, respectively. With these methods, we also show large-scale single-cell analysis of diverse types of live cells (e.g., microbes, cancer cells, blood cells) based on carbohydrates, proteins, chlorophyll, carotenoids, and lipids with unique capabilities that are not possible with fluorescence-based flow cytometry. We also discuss a novel class of opportunities that can be offered by high-throughput vibrational flow cytometry.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11236, Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry, 112360R (9 March 2020); doi: 10.1117/12.2548548
Show Author Affiliations
Keisuke Goda, The Univ. of Tokyo (Japan)
Yasuyuki Ozeki, The Univ. of Tokyo (Japan)
Kotaro Hiramatsu, The Univ. of Tokyo (Japan)
Yuta Suzuki, The Univ. of Tokyo (Japan)

Published in SPIE Proceedings Vol. 11236:
Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry
Wolfgang Petrich; Zhiwei Huang, Editor(s)

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