Share Email Print
cover

Proceedings Paper

Label-free super-resolution microsphere-assisted microscopy of biological samples (Conference Presentation)

Paper Abstract

Most of the recent super-resolution imaging techniques developed for biology are based on fluorescence properties which generally require toxic dyes. We show how super-resolution can be obtained without dyes by simply adding 20-micrometer-diameter dielectric spheres on the sample under a microscope objective. The microsphere behaves as a non-classical lens, collecting evanescent waves in a full-field imaging process. Resolutions of up to between /5 and /7 can be reached in air and in immersion, respectively. For translucent biological samples, a dark-field optical setup is proposed. Performance of the label-free super-resolution technique is demonstrated through the imaging of fixed nerve sections from a mouse embryo brain.

Paper Details

Date Published: 10 March 2020
PDF
Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 1125112 (10 March 2020); doi: 10.1117/12.2548540
Show Author Affiliations
Sylvain Lecler, Lab. des sciences de l'Ingénieur, de l'Informatique et de l'Imagerie (France)
Institut National des Sciences Appliquées de Strasbourg (France)
Stéphane Perrin, Lab. des sciences de l'Ingénieur, de l'Informatique et de l'Imagerie, CNRS (France)
Giorgio Quaranta, Ctr. Suisse d'Electronique et de Microtechnique SA (Switzerland)
Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Nadia Messaddeq, Institut de génétique et de biologie moléculaire et cellulaire (France)
Univ. de Strasbourg (France)
Institut National de la Santé et de la Recherche Médicale (France)
Nicolas Lemercier, Institut de génétique et de biologie moléculaire et cellulaire (France)
Univ. de Strasbourg (France)
Institut National de la Santé et de la Recherche Médicale (France)
Jean-Luc Vonesch, Institut de génétique et de biologie moléculaire et cellulaire (France)
Univ. de Strasbourg (France)
Institut National de la Santé et de la Recherche Médicale (France)
Paul Montgomery, Lab. des sciences de l'Ingénieur, de l'Informatique et de l'Imagerie, CNRS (France)
Univ. de Strasbourg (France)


Published in SPIE Proceedings Vol. 11251:
Label-free Biomedical Imaging and Sensing (LBIS) 2020
Natan T. Shaked; Oliver Hayden, Editor(s)

© SPIE. Terms of Use
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray