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Proceedings Paper

Tracking bacteria clearance using gold nanoparticles for wound therapy applications
Author(s): Menachem Motiei; Oshra Betzer; Tamar Sadan; Neta Zilony; Guy Topaz; Rachela Popovtzer; Moris Topaz; Dror Fixler
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Paper Abstract

Bacterial infection and biofilm buildup is a major challenge for wound healing. In the present work we developed a method to track bacteria in vivo in order to analyze the progress of the healing. Regulated irrigation combined with negative pressure (RI-NPT) is a nonpharmaceutical therapeutic strategy for reducing bacterial load in acute and chronically infected wounds. Here, we analyzed RI-NPT hydrokinetics and efficacy of bacterial load reduction in wounds. Escherichia coli were loaded with gold nanoparticles, in order to make them "visible" and trackable using computed tomography (CT). Bacteria were tracked using CT in a low-flow rat wound model over time. CT tracking revealed that while regulated negative pressure-assisted wound therapy reduced bacterial load to a limited extent (5%), RI-NPT significantly increased bacterial outflow and clearance (by 45%). This nanotechnology-based approach demonstrates the ability to track bacteria in vivo at real time and also the ability of the RI-NPT technology to effectively reduce bacterial load.

Paper Details

Date Published: 21 February 2020
PDF: 9 pages
Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 112541H (21 February 2020); doi: 10.1117/12.2548441
Show Author Affiliations
Menachem Motiei, Bar-Ilan Univ. (Israel)
Oshra Betzer, Bar-Ilan Univ. (Israel)
Tamar Sadan, Bar-Ilan Univ. (Israel)
Neta Zilony, Bar-Ilan Univ. (Israel)
Guy Topaz, Meir Medical Ctr. (Israel)
Tel Aviv Univ. (Israel)
Rachela Popovtzer, Bar-Ilan Univ. (Israel)
Moris Topaz, Meir Medical Ctr. (Israel)
Tel Aviv Univ. (Israel)
Dror Fixler, Bar-Ilan Univ. (Israel)


Published in SPIE Proceedings Vol. 11254:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII
Dror Fixler; Ewa M. Goldys, Editor(s)

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