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Proceedings Paper

FluorATOM: high-throughput imaging flow cytometry for synchronized biophysical and biomolecular phenotyping (Conference Presentation)

Paper Abstract

Multiplexed asymmetric-detection time-stretch optical microscopy (multi-ATOM) has recently been developed to enable high-throughput quantitative phase imaging flow cytometry, from which single-cell biophysical properties can be measured at large scale. However, it lacks the ability to link such biophysical knowledge to biomolecular signatures at the single-cell precision for validation and correlative multi-scale single-cell analysis. We report a high-throughput multimodal system that integrates multi-ATOM with multiplexed 1-D fluorescence imaging/detection, termed FluorATOM; and applied it to perform synchronized biophysical and biomolecular phenotyping of rare breast circulating tumor cells detected in peripheral blood in a mouse xenograft at a throughput of >10,000 cell/sec.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11250, High-Speed Biomedical Imaging and Spectroscopy V, 112500L (10 March 2020); doi: 10.1117/12.2548252
Show Author Affiliations
Kelvin Lee, The Univ. of Hong Kong (Hong Kong, China)
Maolin Wang, The Univ. of Hong Kong (Hong Kong, China)
Isabella Cheuk, The Univ. of Hong Kong (Hong Kong, China)
Vivian Shine, The Univ. of Hong Kong (Hong Kong, China)
Ava Kwong, The Univ. of Hong Kong (Hong Kong, China)
Kenneth Wong, The Univ. of Hong Kong (Hong Kong, China)
Hayden So, The Univ. of Hong Kong (Hong Kong, China)
Kevin Tsia, The Univ. of Hong Kong (Hong Kong, China)


Published in SPIE Proceedings Vol. 11250:
High-Speed Biomedical Imaging and Spectroscopy V
Kevin K. Tsia; Keisuke Goda, Editor(s)

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