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Proceedings Paper

Epi-mode tomographic quantitative phase imaging in thick scattering samples
Author(s): Francisco E. Robles
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Paper Abstract

Quantitative phase imaging (QPI) is an important tool in biomedicine that yields label-free access to cellular and subcellular structures with nanometer-scale sensitivity. However, implementation of QPI involves a transmissionbased geometry and requires thin samples, severely hindering its overall utility in biomedicine. Here we describe our recently developed method, quantitative oblique back illumination microscopy (qOBM), which overcomes this significant limitation and achieves epi-mode, tomographic, quantitative phase imaging in thick samples, including intact thick tissues. Here we describe the method in detail, show validation experiments and results from thick scattering samples.

Paper Details

Date Published: 20 February 2020
PDF: 5 pages
Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511Y (20 February 2020); doi: 10.1117/12.2547693
Show Author Affiliations
Francisco E. Robles, Georgia Institute of Technology & Emory Univ. School of Medicine (United States)

Published in SPIE Proceedings Vol. 11251:
Label-free Biomedical Imaging and Sensing (LBIS) 2020
Natan T. Shaked; Oliver Hayden, Editor(s)

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