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Proceedings Paper

Long-term Brillouin imaging of live cells with reduced photodamage at 660nm wavelength reveals cell mechanical response to environmental cues (Conference Presentation)
Author(s): Milos Nikolic; Kandice Tanner; Giuliano Scarcelli

Paper Abstract

In Brillouin microscopy, absorption-induced photodamage of incident light is the primary limitation on signal-to-noise ratio in many practical scenarios. 660 nm may represent an optimal wavelength for Brillouin microscopy as it offers minimal absorption-mediated photodamage at high Brillouin scattering efficiency. We demonstrate that live cells are ~80 times less susceptible to the 660 nm incident light compared to 532 nm light, which overall allows Brillouin imaging with more than 30 times higher signal intensity. We apply this improved Brillouin microscope to analyze the response of human glioblastoma cells to a range of in vitro biomimetic environments.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11242, Optical Elastography and Tissue Biomechanics VII, 112420K (9 March 2020); doi: 10.1117/12.2547661
Show Author Affiliations
Milos Nikolic, Univ. of Maryland, College Park (United States)
Kandice Tanner, National Cancer Institute (United States)
Giuliano Scarcelli, Univ. of Maryland, College Park (United States)

Published in SPIE Proceedings Vol. 11242:
Optical Elastography and Tissue Biomechanics VII
Kirill V. Larin; Giuliano Scarcelli, Editor(s)

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