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Proceedings Paper

Raman hyperspectral imaging of transferrin-bound iron in T47D and MDA-MB-231 breast cancer cells
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Paper Abstract

Iron is an essential element required for human life, and is highly regulated in the body. Iron deficiency leads to many adverse health effects, such as anemias. The exact mechanisms of iron release in cells are not well known. We developed a Raman micro-spectroscopy technique that allows detection of transferrin (Tf) bound iron inside intact human cells. Ferric iron (Fe3+) bound to serum Tf is internalized into cells via the transferrin receptor (TfR). Methods that allow determining when and where Tf releases iron inside a cell lead to a better understanding of disease progression, including cancer. We have previously shown that Raman micro-spectroscopy is able to detect and quantify the Tf-bound iron in human breast cancer T47D cells. In this work, we applied hyperspectral Raman imaging to visualize the spatial distribution of Tf-bound iron in human breast cancer T47D and MDAMB231 cells internalized with iron-loaded Tf. We have also shown that Raman imaging can quantify the amount of iron under different times of Tf internalization prior to fixation. Raman microspectroscopy provides a unique way to determine the amount of iron under different Tf internalization times by employing the Raman metric, which was used to quantify iron content in iron bound Tf (holo-Tf) samples. Importantly, Raman microspectroscopy can be used to follow iron release from Tf in breast cancer cells. Determining the kinetics and location of iron release in cancer cells is key to further our understanding of iron metabolism during cancer progression.

Paper Details

Date Published: 20 February 2020
PDF: 6 pages
Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511E (20 February 2020); doi: 10.1117/12.2547638
Show Author Affiliations
Ting Chean Khoo, Univ. at Albany (United States)
Kate Tubbesing, Albany Medical College (United States)
Alena Rudkouskaya, Albany Medical College (United States)
Georgios Athanassiadis, Univ. at Albany (United States)
Anna Sharikova, Univ. at Albany (United States)
Margarida Barroso, Albany Medical College (United States)
Alexander Khmaladze, Univ. at Albany (United States)

Published in SPIE Proceedings Vol. 11251:
Label-free Biomedical Imaging and Sensing (LBIS) 2020
Natan T. Shaked; Oliver Hayden, Editor(s)

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