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Proceedings Paper

Imaging neuronal and astrocytic Ca2+ transients and hemodynamic responses evoked by single stimulation in rodent cortex
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Paper Abstract

As transient intracellular Ca2+ changes play an important role in many essential processes including neuronal and astrocytic plasticity, tracking brain activity via Ca2+ is crucial. Unlike hemodynamics, Ca2+ change must be measured optically using an ionic fluorescent Ca2+ indicator. Here, we combine our highly sensitive multimodality optical imaging platform with genetically encoded Ca2+ indicator (GCaMP6f) expressed in neurons or astrocytes in somatosensory cortex, which enables simultaneous tracking of single-stimulation-evoked neuronal, astrocytic Ca2+ transients along with the corresponding hemodynamic responses at high spatiotemporal resolutions. We imaged neuronal and astrocytic Ca2+ transients from mouse cortex in response to a single electrical pulse (3mA, 0.3ms). Our results show that the neuronal Ca2+ responses were strong (ΔF/FN=6.4±0.29%), fast (latency τN=6±2.7ms) and of short duration (ΔtN=537±34ms) whereas the astrocyte responses were weak, slow and long-lasting (i.e., ΔF/FA=1.7±0.1%, τA=313±65ms, ΔtA =993±48ms). The synchronized activities among astrocytes were temporally less correlated than those among neurons. These results demonstrate the capability of optical detection of cell-specific Ca2+ activities from synchronized neuronal, astrocyte ensembles concurrently with the hemodynamic responses within the neuro-glio-vascular network, which can facilitate the study of the roles of astrocytes in the neurovascular coupling process. We also report time-lapse image results to analyze the interactions between stimuli-evoked neurovascular response versus the spontaneous cortical slow oscillations for brain functional studies

Paper Details

Date Published: 21 February 2020
PDF: 5 pages
Proc. SPIE 11226, Neural Imaging and Sensing 2020, 1122617 (21 February 2020); doi: 10.1117/12.2547621
Show Author Affiliations
Wei Chen, Stony Brook Univ. (United States)
Kicheon Park, Stony Brook Univ. (United States)
Xiaochun Gu, Stony Brook Univ. (United States)
Yingtian Pan, Stony Brook Univ. (United States)
Congwu Du, Stony Brook Univ. (United States)

Published in SPIE Proceedings Vol. 11226:
Neural Imaging and Sensing 2020
Qingming Luo; Jun Ding; Ling Fu, Editor(s)

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