The work is devoted to determining the diffusion coefficient of methylene blue in human gum tissue in vitro using spectroscopic methods. The calculations are based on the application of the free diffusion model, the second Fick law, and the modified Bouguer – Lambert – Beer law. For the first time, the diffusion coefficient of methylene blue into the tissue of the human gingival mucosa in vitro was determined, which, by averaging 10 samples is (1.26±0.34)·10^–7 cm²/s. It was revealed that after the gum is completely stained with dye, the light does not pass into the biological tissue from 200-699 nm, which includes the areas of dye absorption peaks necessary for photoactivation of the dye during photodynamic therapy. The results obtained are important for the personified choice of this method in vivo in the treatment of superficial pathological localizations.

Date Published: 19 February 2020
PDF: 7 pages
Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 1122315 (19 February 2020); doi: 10.1117/12.2547577

Published in SPIE Proceedings Vol. 11223:
Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
Tianhong Dai; Jürgen Popp; Mei X. Wu M.D., Editor(s)