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Proceedings Paper

Pathological crystal imaging using computational polarized light microscopy (Conference Presentation)
Author(s): Bijie Bai; Hongda Wang; Tairan Liu; Yair Rivenson; John Fitzgerald; Aydogan Ozcan

Paper Abstract

We report a single-shot computational polarized-light microscopy (SCPLM) method for identifying pathological crystals in bodily-fluids. We utilize the four-directional polarizers integrated on the pixels of a CMOS image sensor to reconstruct the transmittance, retardance, and slow-axis orientation maps of the objects with a single image exposure. Using SCPLM, we imaged birefringent crystals found in synovial fluid, e.g., monosodium urate, calcium pyrophosphate dehydrate, and triamcinolone acetonide. The quantitative birefringence images created by our method are pseudo-colored and digitally-integrated with bright-field images to highlight the birefringent crystals within the background. We believe this single-shot, quantitative, and easy-to-operate method will significantly benefit rheumatology.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430F (9 March 2020); doi: 10.1117/12.2547403
Show Author Affiliations
Bijie Bai, Univ. of California, Los Angeles (United States)
Hongda Wang, Univ. of California, Los Angeles (United States)
Tairan Liu, Univ. of California, Los Angeles (United States)
Yair Rivenson, Univ. of California, Los Angeles (United States)
John Fitzgerald, Univ. of California, Los Angeles (United States)
Aydogan Ozcan, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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