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Proceedings Paper

Cytoplasmic protein imaging with mid-infrared photothermal microscopy (Conference Presentation)

Paper Abstract

As an alternative imaging technique to conventional IR microscopy, a mid-infrared photothermal microscopy has been suggested to achieve spatial resolution at the submicrometer level and the inherent chemical contrast upon vibrational excitation. It also has substantial potential for real-time imaging of live organisms to observe the cellular dynamics without photodamage or photobleaching of fluorescent labels. We performed real-time imaging of oligodendrocytes to investigate cellular dynamics and observed a photothermal contrast associated with traveling protein complexes on an axon of live neutrons. We anticipate that mid-infrared photothermal imaging will be of great use for gaining insights into the field of biophysical science, especially with regard to cellular dynamics and functions.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112520Z (10 March 2020); doi: 10.1117/12.2547269
Show Author Affiliations
Jong Min Lim, Institute for Basic Science (Korea, Republic of)
Chanjong Park, Institute for Basic Science (Korea, Republic of)
Korea Univ. (Korea, Republic of)
Minhaeng Cho, Institute for Basic Science (Korea, Republic of)
Korea Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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