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Proceedings Paper

In vivo cuticle intact Drosophila mushroom body imaging using laser scanning optical resolution photoacoustic microscopy (Conference Presentation)
Author(s): Kai-Yao Chang; Wen-Hung Shih; Shun-Chi Wu; Kwok Ho Lam; Yen-Yin Lin; Ann-Shyn Chiang; Meng-Lin Li

Paper Abstract

Surgical removal of the head cuticle of Drosophila is necessary for optical brain imaging, leading to the Drosophila death in a short period of time and thus hindering long term monitoring. Targeting to the unmet need of surgery free procedure for Drosophila brain researches, in this study, laser scanning optical resolution photoacoustic microscopy (LSOR-PAM) of in vivo cuticle intact RCaMP labeled Drosophila mushroom body (MB) is presented. The MB is a higher-order olfactory center in Drosophila brain. This study paves the way toward exploring LSOR-PAM functional imaging capability of cuticle intact Drosophila brain under olfactory stimulus.

Paper Details

Date Published: 6 March 2020
Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 112401Z (6 March 2020); doi: 10.1117/12.2547187
Show Author Affiliations
Kai-Yao Chang, National Tsing Hua Univ. (Taiwan)
Wen-Hung Shih, National Tsing Hua Univ. (Taiwan)
Shun-Chi Wu, National Tsing Hua Univ. (Taiwan)
Kwok Ho Lam, The Hong Kong Polytechnic Univ. (China)
Yen-Yin Lin, National Tsing Hua Univ. (Taiwan)
Ann-Shyn Chiang, National Tsing Hua Univ. (Taiwan)
Meng-Lin Li, National Tsing Hua Univ. (Taiwan)

Published in SPIE Proceedings Vol. 11240:
Photons Plus Ultrasound: Imaging and Sensing 2020
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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